Inactivated sendai virus

WebPreparation of Sendai Virus for Cell Fusion. Reet Tees, in Immunological Methods, 1979. V ASSAY OF INFECTIVITY. The inactivated virus is diluted in tenfold steps (10 −1 to 10 −6) in Earle's BSS and 0.05 ml of each dilution is inoculated allantoically into a group of three eggs (see Section I). After incubation at 35°C for 68–72 hr, about ... WebJul 18, 2016 · Some studies [37–38] have found that UV-inactivated Sendai virus is different from a saline control, which is unable to replicate in cells because the genome is destroyed by UV irradiation, but still contains the complete structure of the live HVJ envelope containing F, HN, and M proteins. These proteins have displayed antitumor effect ...

Systemic Administration of Platelets Incorporating …

WebAug 26, 2024 · Inactivated Sendai virus strain Tianjin induces apoptosis and autophagy through reactive oxygen species production in osteosarcoma MG-63 cells Zhe Han, Qing … WebDec 1, 2014 · We previously reported that inactivated Sendai virus (hemagglutinating virus of Japan; HVJ) envelope (HVJ-E) activates anti-tumor immunity and induces cancer cell-selective apoptosis. The hemagglutination activity that blocks the systemic administration of HVJ-E was dramatically attenuated by incorporation into platelets. Platelets ... shape of all planets https://davidlarmstrong.com

Inactivated Sendai virus strain Tianjin induces apoptosis and …

WebThe close apposition of infected and uninfected cells occurring during the agglutination pro- duced by treatment with ultraviolet-inactivated Sendai virus in the cold, rather than complete cell ... WebSinovac Inactivated Inactivated 2 0, 14 days IM NCT04383574 NCT04352608 NCT04456595 669/UN6.KEP/EC/2024 ... Inactivated Inactivated whole virus National Research Centre, Egypt SARS-CoV2 Pre-Clinical ... Non-Replicating Viral Vector Sendai virus vector ID Pharma SARS-CoV2 Pre-Clinical WebFeb 23, 2024 · Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res. 67 (1), 227–236. Article CAS PubMed Google Scholar Fujihara A., Kurooka M., Miki T., Kaneda Y. 2008. Intratumoral injection of inactivated Sendai virus particles elicits strong antitumor activity by … pontymister angling club

Systemic Administration of Platelets Incorporating Inactivated Sendai …

Category:Full article: Oncolytic Sendai virus-based virotherapy for cancer ...

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Inactivated sendai virus

Systemic Administration of Platelets Incorporating …

Webfrom publication: Enhancement of Sendai virus-mediated cell fusion by cupric ions The effect of divalent cations on cell fusion by concentrated Sendai virus, inactivated by beta-propiolactone ... WebSendai virus (SeV) is a non-segment negative-sense RNA virus that naturally infects and causes pneumonia in mice. As a prototypic member of the family Paramyxoviridae, SeV has been characterized well, and these studies revealed numerous traits of paramyxovirus biology. The reverse genetics system to rescue SeV was first established in 1995.

Inactivated sendai virus

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WebSep 5, 2013 · Sendai virus strain Tianjin was isolated from the lungs of marmoset and proved to be a novel genotype of Sendai virus. In this study, we explored the antitumor …

WebDec 8, 2015 · Inactivated Sendai virus (hemagglutinating virus of Japan; HVJ) envelope (HVJ-E) induces anticancer immunity and cancer cell-selective apoptosis through the recognition of viral RNA genome fragments by retinoic acid-inducible gene-I (RIG-I). Here, we discovered that the “copy-back” type of defective-interfering (DI) particles that exist in ... WebMar 15, 2024 · Justia Patents Paramyxoviridae (e.g., Parainfluenza Virus, Respiratory Syncytial Virus, Rinderpest Virus, Sendai Virus, Canine Tracheobronchitis Virus, Turkey ... :3987-94). Heat-inactivated antisera was initially diluted in 10% FBS-DMEM at 1:40 (pre-challenge serum) or 1:20 (post-challenge serum) dilutions, then 2-fold serially diluted in a …

WebDec 21, 2024 · Inactivated-Sendai virus: UV-irradiated Sendai virus particle (HVJ-E) – Induction of apoptosis in cancer cells by introduction of viral RNA genome fragments into cytoplasm – Induction of necroptosis in cancer cell lack caspase-8 by HVJ-E membrane fusion – Activation of antitumor immunity (DCs stimulation, NK cells activation, Treg ... HVJ-E was manufactured at GenomIdea Inc. (Osaka, Japan). HVJ was irradiated by the treatment with β-propiolactone and ultraviolet irradiation, which caused the alkylation and the fragmentation of the RNA genome. HVJ-E was purified by four steps of column chromatography, stabilized by lyophilization and … See more This study was a single-arm, open-label, single-center, phase I/II study of HVJ-E in patients with CRPC who were docetaxel resistant or could not receive docetaxel treatments. The primary end points were to evaluate safety and … See more Eligible patients had to have CRPC and be docetaxel resistant or unable to receive docetaxel treatment and have a prostate in which HVJ-E could be administered. Patients were … See more Patients were monitored in hospital from day 1 to day 14 and at each outpatient visit on days 21 and 28 of each cycle. The National Cancer Institute’s Common Terminology Criteria for Adverse Events version 4.0 was used … See more A 3+3 design was used to determine the maximum tolerated dose of HVJ-E administration. HVJ-E was administered directly to the … See more

WebMultinucleate cell formation induced by beta-propiolactone-inactivated Sendai virus in human fibroblast cells (a system in which fusion results from an interaction of the viral …

WebOct 26, 2024 · Sendai virus (SeV), a prototype of the Paramyxoviridae family, binds to the host cell surface through its hemagglutinin-neuraminidase glycoprotein (HN). This binding also triggers the fusion protein (F), another envelope glycoprotein to undergo a conformational change and catalyze the mixing of viral and cellular lipid membranes ( … shape of a logistic curveWebDec 4, 2024 · In recent years, inactivated Sendai virus particles (hemagglutinating virus of Japan envelope, HVJ-E) have been shown to contribute to several anti-cancer effects, such as the activation of anti-tumor immunity via anti-tumorigenic neutrophils in the tumor microenvironment , the suppression of murine melanoma growth by host immune … pontypinna hideawaysWebAug 26, 2024 · Sendai virus strain Tianjin, a novel genotype of Sendai virus, has been proven to possess potent antitumor effect on certain cancer cell types although inactivated by ultraviolet (UV). This study was carried out to investigate the in vitro anticancer properties of UV-inactivated Sendai virus strain Tianjin (UV-Tianjin) on human osteosarcoma ... shape of a magnetic fieldWebDec 4, 2024 · In recent years, inactivated Sendai virus particles (hemagglutinating virus of Japan envelope, HVJ-E) have been shown to contribute to several anti-cancer effects, … pontypandy wales mapWebMurine respirovirus, formerly Sendai virus (SeV) and previously also known as murine parainfluenza virus type 1 or hemagglutinating virus of Japan (HVJ), is an enveloped, 150-200 nm–diameter, negative sense, single-stranded RNA virus of the family Paramyxoviridae. It typically infects rodents and it is not pathogenic for humans or domestic animals pon type aWebJan 8, 2007 · Abstract. UV-inactivated, replication-defective Sendai virus particles [hemagglutinating virus of Japan envelope (HVJ-E)] injected into murine colon carcinoma (CT26) tumors growing in syngeneic BALB/c mice eradicated 60% to 80% of the tumors and obviously inhibited the growth of the remainder. Induced adaptive antitumor immune … pontypoolWebJan 8, 2007 · Inactivated Sendai virus particles [hemagglutinating virus of Japan envelope (HVJ-E)] are safe and efficient nonviral vectors for drug delivery as they can incorporate … pontypol old comrades