Web1 aug. 2024 · The Bcl11b flox/flox mice (C57/BL6 background) were generously provided by Mark Leid's lab and the B6N.Cg-Tg (KRT14-cre)1Amc/J (stock number 018964) were … Web20 jun. 2016 · Krt14 homologous arms were PCR-amplified from mouse 129/Sv genomic DNA as template. ... Buch, T. et al. A Cre-inducible diphtheria toxin receptor mediates …
Deciphering a Message from the Nucleus: How Transcription …
Web4 apr. 2024 · Mutation details: This transgene expresses Cre recombinase under the control of a human keratin 14 promoter. This promoter is active in skin, salivary gland and … Web诱导型 Cre-loxP 系统介导了时间特异性基因敲除。. 通过对给予诱导剂的时间的控制,人为操控基因敲除的发生。. 诱导型Cre有哪几种类型?. 通过诱导剂来调节驱动 Cre 重组酶 … number of indian restaurants in usa
3.小鼠实验(条件性敲除,肝癌模型)
Web10 dec. 2024 · We crossed this genetic model with keratin 14 Cre (Krt14-Cre)–expressing mice to generate mice with epidermal deletions of Mll4 (Krt14-Cre; Mll4 fl/fl or “Mll4-eKO”) (fig. S1A). Mll4-eKO mice were born in the expected Mendelian ratios (fig. S1B) with no visible differences from littermate controls during the first week of life. Web24 jun. 2011 · We therefore generated mice in which diphtheria toxin A (DTA) was conditionally expressed in basal cells of stratified epithelia by crossing the ROSA26-tm-DTA mouse (Ivanova et al., 2005) with one having a Tamoxifen-dependent Cre recombinase under the control of the Krt14 promoter (Vasioukhin et al., 1999) (hereafter the DTA … Web30 jul. 2024 · To determine if keratinocyte inhibition affects in vivo heat sensitivity, we performed the Hargreaves radiant heat assay (Hargreaves et al., 1988) on Krt14:Arch Cre+ and Krt14:Arch Cre- mice. Krt14:Arch Cre+ mice exhibited longer withdrawal latencies to the heat stimulus when 590 nm light was applied to the stimulated hindpaw . nintendo switch pro controller pink