WebArticle Snippet: For live imaging and red nuclear labeling, 1205Lu cells were transduced with NucLight Red Lentivirus Reagent ( Essen Bioscience) and selected with puromycin (1 μg/ml, Sigma‐Aldrich). Techniques: Imaging, Labeling, Derivative Assay, Staining, Flow Cytometry, Cell Culture, Western Blot. WebNucLight nuclear labeling reagents or labeled cell lines for multiplexed measurements of proliferation and apoptosis in the same well. IncuCyte® Apoptosis Assay For the …
IncuCyte Apoptosis Assay - Labettor
Web1 jan. 2014 · The NucLight Red reagent is a lentivirus-based reagent which stably integrates into the host cell line and labels the nuclei in a non-perturbing way (Fig. 1a). The caspase-3/7 reagent is an inert, non-fluorescent substrate that freely crosses the cell membrane and is cleaved by activated caspase-3/7 resulting in the release of a green … WebIncuCyte® NucLight Rapid Red Reagent for nuclear labeling (Cat. No. 4717) The IncuCyte NucLight Rapid Red Reagent for nuclear labeling is a cell permeable DNA stain that specifically stains nuclei in cells and is ideally suited to the mix-and-read, real-time quantification using the IncuCyte® Live-Cell Analysis System. More information. smtp paging service
Incucyte® Nuclight Reagents Cell Proliferation - Essen …
WebI plan to assay proliferation dynamics of U-2 OS cells using the IncuCyte platform in a 96 well format. I will be using the IncuCyte NucLight Rapid Red ( WebThe Incucyte® Nuclight live-cell labeling reagents are ideal for use in cell counting and proliferation studies and can be easily multiplexed with other Incucyte® Cell Health … Additional Training. Sartorius provides basic instrument training as part of all new … Assess cell health, proliferation and viability. Examine cell function, cellular … WebIncucyte ® NucLight Lentivirus Reagents provide homogenous expression of a nuclear-restricted GFP (green fluorescent protein) or mKate2 (red fluorescent protein) in your … smtp path